Veterinary Microbes

Rumen Microbes

Dairy Microbes

Ø      Exploration and collection of microbes of Veterinary origin / significance / relevance;

Ø      To identify and characterize the isolated / collected microbial pathogens viz. viruses, bacteria, fungi, mycoplasma, protozoa etc. isolated from different disease conditions and carrier animals by conventional and molecular techniques.

Ø      Collection and central storage of animal microbes from existing culture collection centers, institutions and universities.

Ø      To clone and sequence important genes to generate epidemiological information and to understand disease pathogenesis

Ø      To create a genome/gene bank of different microbes by storing genomic DNA/RNA, recombinant plasmid clones, transfected cell lines, transformed bacteria etc for conservation of microbial biodiversity

Ø      To procure and reposit reference microbial strains/isolates, vaccine strains,plasmids, genomic DNA/RNA, clones etc

Ø      To reposition, supply and use and /or other biological materials for validating various assays.

Ø      To identify suitable isolate/strains for development of vaccines

Ø      Documentation and digitization of microbial database of cultures of veterinary microbes;

Ø      To isolate cellulolytic rumen bacteria  from Indian cattle, sheep, goats and buffalos

Ø      To isolate rumen fungi from domesticated and wild animals

Ø      To determine the best fibre digesting organisms

Ø      To clone the cellulase gene from the best fibre digesting organisms into a suitable target bacteria.

Ø      To isolate indigenous strains of lactic acid bacteria including the ones with probiotic attributes from ethnic fermented milks and other foods prepared in various parts of India

Ø      To identify and characterize the isolated strains using conventional and molecular techniques based on 16S rRNA sequencing, PCR (Real time), DNA probes and Molecular Beacons / DNA Micro-assay.

Ø      To analyse the plasmid pool encoding commercially important traits of selected cultures and their characterization at molecular level.

Ø      To construct new recombinants with different phenotypes by gene transfer systems

Ø      To clone and sequence commercially important genes of lactic acid bacteria particularly related to probiotic functions

Ø      To identify the commercial potential of these diversified strains for designing novel starter cultures with functional / probiotic properties

Ø      To conserve these strains and their germplasm ex situ and create a catalogued National culture bank of indigenous lactic strains at NDRI.

Veterinary Microbes

Rumen Microbes

Dairy Microbes

Ø      The programme would generate useful information on the association of various pathogenic strains with disease conditions.

Ø      A repository will be created as a common source of important pathogens and their use for various research and development oriented activities

Ø      In the long term, the immunobiologicals and diagnostic so developed could be patented and the technology would be commercialized.

Ø      Resource generation through the sale of microbial strains, genes, plasmids etc.

Ø      Collection of ruminal microorganisms (bacteria and anaerobic fungi) with specialized characteristics at one designated research centre i.e. NDRI, Karnal in different types of formulations (e.g. dust, powder, paste, capsule, syrup etc.) and also as pure cultures /germplasm for R & D work.

Ø      Strategies will be developed that will improve fiber digestion, reduce methane production and detoxify anti-nutritional components of feeds from different sources in India.

Ø      Development and applications of molecular techniques for identifying important rumen microflora and improved knowledge of microbial ecology of rumen.

Ø      Dissemination of knowledge to research and industry through published results/ websites.

Ø      Isolation of a large number (>500 strains) of diversified indigenous strains of lactic acid bacteria.

Ø      Characterization of isolated strains for their potential commercial traits using phenotypic, biochemical & physiological tests and molecular biology techniques to their respective taxons and even strain level.

Ø      These strains will be preserved ex-situ in freeze-dried state

Ø      Development of a well structured and catalogued national consortium of defined and thoroughly characterized indigenous lactic starter cultures and their germplasm retrieved from different regions of the country at NDRI which will cater to the requirements of authentic dairy cultures and research strains for various industrial and academic institutions in the country.